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KMID : 0545119910010040274
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 4 p.274 ~ p.280
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Effect of IPTG Induction on Production of β-Galactosidae-PreS2 Fusion Protein in Recombinant Escherichia coli
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Nam Soo-Wan
Park Young-Hoon
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Abstract
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Effects of IPTG induction on cell growth and production of β-galactosidase-preS2 fusion protein (βgal-preS2) were studied in a defined medium using a recombinant Escherichia coli JM109/ pCMHB30. IPTG was added (0.2mM) to induce the cloned-gene expression in the early-, mid-, and late-log growth phases. The most serious decreases in growth rate and plasmid stability were observed for the induction in the early-log growth phase. The expression level of βgal-preS2 attained by the induction in the mid-log phase was about 0.51㎎ fusion protein/㎎ total cellular protein, which was 2- and 5-fold improvement over the levels obtained with the induction in the early- and late-log phases. Formation of acidic byproducts including acetate and pyruvate showed different profiles during the fermentation period for each cases of induction; pyruvate was the major byproduct for the induction in the early-log phase while acetate production became more significant for the cases of induction in the mid- and late-log phases.
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